cell culture human umbilical vein endothelial cells huvecs  (ATCC)

Journal: Journal of Biological Engineering

Article Title: Anti-CD31 antibody preconditioning for enhancement of endothelial cell capture and vascularization: a novel strategy for bioengineering lung scaffolds

doi: 10.1186/s13036-025-00593-x

Figure Lengend Snippet: Effect of coating agents on the attachment and growth of Endothelial cells (ECs) on precision cut lung slices (PCLS). ( A ) Schematic diagram describes the experimental design of each assay using decellularized PCLS. ( B , C ) Coating of decellularized scaffolds enhance the attachment of Hoechst 33,342-labeled (blue) mC166 cells on PCLS compared to the uncoated group. Each group n = 6. ( D ) Quantification of EC cell metabolic activity using cell growth assay using Cell Counting Kit-8 (CCK-8) revealed that precoating of PCLS increases ECs growth and metabolic activity (Optical Density (OD) measured at Day 1, 3 and 5) compared to ECs cultured on uncoated PCLS. Each group n = 6. ( E ) Quantification of the attachment of Hoechst-labeled HUVECs on anti-CD31 coated PCLS coated with 12.5, 25, 50, or 100 µg/mL anti-CD31 Ab. Results represent the mean of measurements taken from 3 PCLS seeded with HUVEC cells from 3 independent experiments. Each group n = 3. ( F ) Quantification of HUVEC cell metabolic activity using cell growth assay using Cell Counting Kit-8 (CCK-8) revealed that precoating of PCLS increases ECs growth and metabolic activity (Optical Density (OD) measured at Day 1, 3 and 5) compared to ECs cultured on uncoated PCLS. Each group n = 3. Scale bar = 200 μm. The results represent the mean of measurements taken from 3 technical replicates. Each number represents a biological replicate (independent experiment). M ean ± SD, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: Endothelial cell growth medium (EGM) (Lonza Bioscience, MD, USA) was used to culture human umbilical vein endothelial cells (HUVEC) (ATCC, Manassas, VA).

Techniques: Labeling, Activity Assay, Growth Assay, Cell Counting, CCK-8 Assay, Cell Culture

Journal: Bioactive Materials

Article Title: Self-assembled hybrid hydrogel microspheres create a bone marrow-mimicking niche for bone regeneration

doi: 10.1016/j.bioactmat.2025.08.003

Figure Lengend Snippet: Angiogenesis effect of the self-assembled hybrid microspheres-based BM-mimicking niche. A-E) Results from the groups without BMSCs: A, B) Representative Transwell migration images and the quantitative assessment of HUVECs numbers based on the crystal violet staining results on day 1. C, D) Representative scratch assay images and the quantitative analysis of migration rate of HUVECs on day 1. E-F) Representative images and quantitative assessment of tube-like structure formation of HUVECs induced by released peptides at 6 h. G-H) Representative images and quantitative assessment of tube-like structure formation of HUVECs cultured in the conditioned medium from surface-adhesive BMSCs at 6 h. I) Expression of angiogenesis-related genes of HUVECs cultured in the conditioned medium from surface-adhesive BMSCs (VEGF, PDGF, FGF, CD31, α-SMA, and eNOS) on day 3. J-K) Representative images and quantitative assessment of tube-like structure formation of HUVECs cultured in the conditioned medium from encapsulated BMSCs at 6 h. L) Angiogenesis-related gene expression of HUVECs cultured in the conditioned medium from encapsulated BMSC on day 3. (ns = no significant difference, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, n = 3).

Article Snippet: Human umbilical vein endothelial cells (HUVECs, Procell, China) were cultured in the HUVECs complete culture medium (Procell, China) at 37 °C with 5 % CO 2 .

Techniques: Migration, Staining, Wound Healing Assay, Cell Culture, Adhesive, Expressing, Gene Expression